Effects of Lactobacillus Plantarum on the Ensiling Quality of Cauliflower Wastes at Different Temperature

In order to utilize and dispose vegetable wastes (VWs) by ensiling in a timely and safe manner,the aim of this work is to investigate the inoculation of Lactobacillus plantarum (LP) on the ensiling quality of cauliflower wastes at different temperature (20℃,25℃,30℃,35℃,40℃ and 45℃) in terms of organic components,fermentation quality and microbial community.On this basis,the correlation between microbial community and ensiling quality parameters during ensiling were established.The addition of LP can significantly increase the content of dry matter,crude protein and lactic acid accompanied by the decrease of pH and ammonia nitrogen content for the silages.The dry matter,soluble carbohydrate and crude protein contents in LP were significantly higher than those in the control group (no additive,CK) under medium and low temperatures (20~35℃).the contents of lignocellulosic fractions such as neutral detergent fiber,acid detergent fiber and cellulose in LP group were significantly higher than those in CK,which resulted in the significant decrease for relative forage value under high temperatures (40℃ and 45℃).The ratios of lactic acid/acetic acid and lactic acid/total organic acid in LP were lower than those in CK,indicating that the fermentation intensity of lactic acid bacteria in LP was weakened at high temperature.Firmicutes in silages were dominant at phyla,and the abundance of lactic acid bacteria (LAB) in LP was significantly higher than that in CK (except 45℃),especially the abundance of LAB in LP exceeded 90% at 30℃ and 35℃,indicating that a well lactic acid fermentation was formed,which suppressed the undesirable microorganisms.Lactobacillus is the main genus of LAB,and its abundance is negatively correlated with the content of neutral detergent fiber and acidic detergent lignin,and positively correlated with the content of lactic acid.In conclusion,the temperature and Lactobacillus plantarum had a significant effect on the ensiling quality,the ensiling of cauliflower wastes inoculated with lactobacillus plantarum at middle-low temperature could effectively improve the ensiling quality and relative feed value.Therefore,the ensiling disposition of cauliflower wastes at 20~35℃ was recommended preferentially.